Molecular imprinting described as a method utilized to create artificial receptors and antibodies by construction of selective recognition sites in a synthetic polymer can be a promising tool for generating peptide and protein artificial specific recognition sites. These materials, as potential antibody substitutes, have attracted great interest and attention in different fields such as peptide and protein purification and separation, chemical/electrochemical/optical sensors/biosensors, chromatographic stationary phases, and enzyme mimics. This review has focused on fundamentals of molecularly imprinted polymers in terms of selection of molecular template, functional monomer, cross linker, and polymerization format. Furthermore, several applications of peptide/protein-imprinted materials are highlighted and challenges regarding the intrinsic properties of peptide/ protein imprinting have been emphasized.

Walnuts are grown for commercial edible nuts. Generally plant proteins play a significant role in human nutrition because they are responsible for many functional properties of food product. Bioactive peptides from food with health promoting properties have been studied extensively in the recent years. In this work, walnut proteins are extracted and hydrolyzed with different digestive enzymes and obtained peptides were tested for antioxidant and anticancer properties. The proteolytic activity was determined using the o-phthaldialdehyde method. Our study demonstrated that peptides fraction resulted from hydrolysis with digestive enzymes can prevent the growth of HT-29 and MDA-MB231 cell lines by 60 percent utilize MMT test. Nearly in all cases, more antioxidant activity resulted in more anticancer property. Treatment of cancer is highly expensive involving drugs that have adverse side effects and toxicity complications. Cheaper alternative methods using bioactive peptides can have better prospects in the economics of cancer prevention and therapy. These bioactive peptides are acceptable due to their non-toxic nature so they may be considered as a potential treatment for cancer in future.